Design of an engineered Gαq protein for high-yield expression and NMR isotope labeling in E. coli
August 2027
G protein-coupled receptors (GPCRs) are pivotal therapeutic targets that signal through heterotrimeric G proteins. While structural insights into Gαs and Gαi/o subfamilies have advanced significantly,...
New pulse sequence for improving cardiac black-blood and PSIR MRI quality in cases of arrhythmia
May 2027
Most conventional cardiac MRI scans, which rely on electrocardiogram (ECG)-triggered segmented acquisition for k-space data, are highly sensitive to arrhythmia—a vulnerability that can induce severe...
Language reorganization in brain arteriovenous malformations with fiber-based regional functional connectivity
May 2027
Structure and function changes of language function processing in brain arteriovenous malformations (AVMs) have been investigated, while the alterations of brain connectivity remain unexplored. This...
A pseudo-labeling approach for automated assignment of unlabeled proteins exceeding 100 amino acid residues
May 2027
In the era of AI-driven protein structure prediction, there is an urgent demand for rapid, accessible experimental approaches to validate computationally predicted structural models or to improve the...
Solvent-dependent of Pt/MoS2 electrocatalytic activity toward ethanol oxidation reaction studied by in situ electrochemical NMR spectroscopy
February 2027
In this work, MoS2 was prepared as a supporting matrix for Pt via a hydrothermal method using different solvents, and the electrocatalytic activity of Pt/MoS2 for the ethanol oxidation reaction was...
The perspective of artificial intelligence for NMR
February 2027
Nuclear magnetic resonance (NMR) spectroscopy is an indispensable analytical technique in chemistry, biology, and medicine, offering molecular-level structural and dynamic insights for metabolite identification...
Predictions and cross-center validation of IDH status and 1p/19q status in adult diffuse glioma with non-invasive magnetic resonance spectroscopy
February 2027
This study developed and validated a non-invasive multi-metabolite magnetic resonance spectroscopy framework for preoperative molecular subtyping of adult diffuse gliomas. Using a cross-center, cross-vendor...
Real-time NMR analysis of glutamine metabolism for screening anti-cancer agents in living cells
February 2027
Metabolic reprogramming is a substantial obstacle for anticancer drug screening, as targeted therapeutics often lose efficiency due to the dynamic adaption of cancer cells. Glutamine metabolism in cancer...
Applications and future development of solid-state nuclear magnetic resonance for investigating metal-organic frameworks
February 2027
Metal-organic frameworks (MOFs) are a class of porous materials that feature permanent porosity, high crystallinity, and diverse structures. The 2025 Nobel Prize in Chemistry was awarded to three pioneering...
Deciphering the hydrogen shuttle-mediated kinetic memory of Ge-related defects in UV-grade fused quartz via in situ EPR with 3D imaging
February 2027
This study elucidates the photo-induced defect dynamics in standard UV-grade fused quartz (JGS2) by synergizing in situ electron paramagnetic resonance (EPR) spectroscopy with 3D volumetric imaging....
Accurate measurement of longitudinal paramagnetic relaxation enhancement through division after subtraction
February 2027
Paramagnetic relaxation enhancement (PRE) provides valuable distance constraints with respect to unpaired electron for the structural and dynamic characterization of biomolecules. While transverse PRE...
Going high-throughput: Dynamic conformations and interactions in vivo
February 2027
Proteins are intrinsically dynamic molecules that continuously explore conformational ensembles to execute biological functions. Conventional structural biology methods rely on in vitro reconstitution...
Using 19F NMR to quantify enthalpy-entropy compensation in protein-protein interactions
February 2027
Protein structural, stability, and functional equilibria are sensitive to the solution environment and governed by enthalpic and entropic contributions. NMR spectroscopy of suitably labeled- or isotopically...
Elucidating chain conformation in interfacial and crystalline domains of polyethylene-like polyesters by solid-state NMR
November 2026
Design of a fast broadband RF switch for multi-nuclear NMR in lithium-ion batteries
November 2026
To address the need for observing the electrolyte influenced by dissolved metal ions during the charge and discharge process of lithium-ion battery, a fast-switching broadband radio frequency (RF) switch...
Physics-embedded CycleGAN for robust metabolite mapping in short-readout deuterium metabolic imaging
November 2026
Deuterium metabolic imaging (DMI) enables noninvasive, spatially resolved mapping of in vivo metabolism by tracking deuterium-labeled substrates and downstream products, yet it is frequently signal-to-noise-ratio...
Effect of basis choice on quantitative parameter estimation in accelerated subspace reconstructions
November 2026
This work assesses the effects of different simulated and data-driven subspaces on quantitative parameter estimation in highly accelerated subspace reconstructions. Three different methods for generating...
A hybrid deep learning framework based on VGG19 and U-Net for accurate brain tumor segmentation in MRI images
November 2026
Accurate segmentation of brain tumors from magnetic resonance imaging (MRI) is a critical task in neuro-oncology, directly impacting diagnosis, treatment planning, and patient outcomes. This study presents...
In vivo targeted MRS detection of 2-hydroxyglutarate molecules in IDH-mutant gliomas via spin regulation
November 2026
2-hydroxyglutarate (2-HG) is a key metabolic biomarker for identifying IDH-mutant gliomas. Non-invasive and accurate detection of 2-HG is of great significance for the early diagnosis of diseases and...
A low-frequency pulsed EPR spectrometer for hyperfine clock transition measurement
November 2026
Low-frequency electron paramagnetic resonance (EPR) spectroscopy proves large sample space and special selectivity to hyperfine spin related transitions in molecules. For instance, the hyperfine clock...
A designed fusion tag improves soluble expression of nanobody in E. coli
November 2026
Nanobodies are small, single-domain antibody fragments derived from camelid heavy-chain antibodies that offer high stability, strong binding affinity, and excellent potential for therapeutic and diagnostic...
Uncovering a cryptic allosteric hub governing energy landscape plasticity within the Nur77 ligand-binding domain
Available online 15 October 2026
Nur77 (NR4A1) is an orphan nuclear receptor whose allosteric mechanism departs fundamentally from that of classical nuclear receptors. Its ligand-binding pocket is constitutively occupied by endogenous...
A spatio-temporal fingerprints with hierarchical activation refinement for predicting task activation maps from resting-state fMRI
Available online 14 October 2026
Predicting task-induced brain activation maps from resting-state fMRI (rs-fMRI) is an important challenge in computational neuroimaging, with potential applications for studying brain function without...