Efficient expression of full-length triple-helix recombinant type III collagen in Pichia pastoris
January 2027
Type III collagen (COL3A1) is a significant fibrillar collagen abundant in vascular and cutaneous tissues, playing vital roles in wound healing and tissue repair. The growing demand for COL3A1 in biomedical...
Defense-associated reverse transcriptases define an emerging class of noncanonical DNA-writing systems
December 2026
Defense-associated reverse transcriptases (DRTs) are emerging as a mechanistically diverse class of bacterial antiviral enzymes. Rather than acting simply as RNA-to-DNA copying proteins, they generate...
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
Advancing our understanding of photosynthesis: discoveries and insights from aquatic plants
January 2027
Aquatic plants inhabit highly dynamic environments characterized by rapid and extreme fluctuations in CO2 availability, light intensity, hydrodynamics, temperature, and pH. These conditions have driven...
Multi-gene Co-expression systems in E. coli: From single-vector designs to programmable expression platforms
June 2026
Escherichia coli (E. coli) has long served as a versatile workhorse for recombinant protein production. As synthetic biology expands the demand for coordinated expression of multiple genes, co-expression...
EINN: An enzyme-informed neural network guided by an enzyme-constrained genome-scale metabolic model
March 2027
Predicting cellular metabolism from molecular data is a central challenge in systems biology, with direct implications for engineering microbial cell factories. Genome-scale metabolic models (GEMs)...
Systematic enzyme and cofactor engineering for efficient ursolic acid biosynthesis in Yarrowia lipolytica
February 2027
Ursolic acid (UA) is a pharmaceutically valuable pentacyclic triterpenoid, but its microbial production is constrained by inefficient cytochrome P450 catalysis and limited cofactor availability. Here,...
Deep Learning–driven synergistic engineering of PET hydrolase for post-consumer PET depolymerization
January 2027
Enzymatic depolymerization of polyethylene terephthalate (PET) offers a promising route to mitigate the increasingly severe problem of plastic pollution. However, the development of highly efficient...
DNA storage: The future direction for medical cold data storage
June 2025
DNA storage, characterized by its durability, data density, and cost-effectiveness, is a promising solution for managing the increasing data volumes in healthcare....
High-level amyrin production in Yarrowia lipolytica via metabolic and enzyme engineering
February 2027
Amyrins arе pеntacyclic tritеrpеnoids with valuablе pharmacеutical and cosmеtic applications; howеvеr, thеir low abundancе in plants and complеx еxtraction procеssеs hindеr sustainablе supply. Hеrе,...
Ribosome engineering enhances genetic code expansion in Saccharomyces cerevisiae
April 2027
Genetic code expansion enables the site-specific installation of noncanonical amino acids (ncAAs) into proteins, but its limited efficiency in eukaryotes remains a major barrier to broader application....
Lactiplantibacillus plantarum as a multifunctional probiotic for inflammatory bowel disease: Mechanisms, synbiotic strategies, and delivery systems
February 2027
Inflammatory bowel disease (IBD) encompasses a group of chronic inflammatory disorders of the bowel, characterized by complex etiologies that are closely associated with an imbalance in intestinal microbiota,...
Engineering Rubisco to enhance CO2 utilization
March 2024
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a pivotal enzyme that mediates the fixation of CO2. As the most abundant protein on earth, Rubisco has a significant impact on global carbon,...
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects
March 2026
The rising global demand for sustainable protein sources poses critical challenges across food, pharmaceutical, and industrial biotechnology sectors. Microbial expression systems provide scalable and...
Identification of integration site library for the development of plasmid-free microbial cell factory in Bacillus subtilis
January 2027
Bacillus subtilis was widely used for enzyme production and was gradually engineered to biosynthesize value-added chemicals with the development of genetic parts for it. Compared to other genetic parts...
Streptomyces: boost chassis strains for production of invaluable natural products
December 2026
Streptomyces species continue to occupy a pivotal role in natural product biosynthesis, owing to their unparalleled metabolic diversity and genetic adaptability. This review focuses on cutting-edge...
Artificial intelligence catalyzes antimicrobial peptide design
February 2027
With broad-spectrum, low resistance, and multifunctional properties, antimicrobial peptides (AMPs) are promising therapeutic agents against drug-resistant pathogens, yet their discovery and optimization...
Ergothioneine biosynthesis: The present state and future prospect
March 2025
Ergothioneine (ERG), a rare natural thio-histidine derivative with potent antioxidant properties and diverse biological functions, is widely utilized in food processing, cosmetics, pharmaceuticals,...
Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
September 2026
Peptide linkers are critical modulators of function in fusion proteins, a foundational technology in modern bioengineering. However, the rational customization of linkers for specific applications remains...
Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications
December 2026
The eukaryotic cell-free protein synthesis (CFPS) system, endowed with intrinsic post-translational modification capabilities and a complex molecular chaperone network, efficiently synthesizes functional...
Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects
December 2021
l-glutamate family amino acids (GFAAs), consisting of l-glutamate, l-arginine, l-citrulline, l-ornithine, l-proline, l-hydroxyproline, γ-aminobutyric acid, and 5-aminolevulinic acid, are widely applied...
scYeast: a biological-knowledge-guided foundation model on yeast single-cell transcriptomics
February 2027
Though large-scale pre-trained models are vital for foundational cell modeling, most of them focus on human or mouse systems, with less emphasis on model organisms like yeast (Saccharomyces cerevisiae),...
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production
June 2025
Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing...
Metabolic state-driven monitoring and control of abnormal pigment formation in sodium gluconate fermentation by Aspergillus niger
February 2027
Abnormal pigment formation during late-stage fungal fermentation poses a significant challenge to industrial product quality. This study established an integrated framework to understand and control...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...